Oslo residents have taken the wolf couple to heart since they were first discovered in January, naming them Fenris and Freya in a newspaper-run poll.
Traces in the snow found in January indicated that Freya was in heat, and experts said that it was likely that the couple would have puppies in around May.
Automatic wildlife cameras run by the Norwegian Institute for Nature Research (Nina) on 29 September filmed the puppies for the first time.
"We clearly see four wolves in the pictures. Because we know roughly how big wolf puppies are at this time of year we can establish that all four are puppies," Nina's Morten Kjørstad told NRK.
He said that when snow falls in the coming months, the puppies would leave tracks, allowing Nina to know with more certainty how many wolf puppies are now living in the forest.