The Ohio University Marching 110 performed the hit in full regalia at half-time of the match between the Ohio Bobcats and Marshall Thundering Herd from nearby Marshall University.
The stadium announcer began by reciting Ylvis's nonsense lyrics over the tannoy: "Well, the cows go moo and the fish go blub, but there's one sound that no one knows."
Then, in the middle of their rendition, the members of the band broke out into perfectly synchronized dance moves from the song's Youtube video.
"It is strange to see so many other people in the world engaging with it," Bård Ylvisaker, one of the two brothers behind the hit told NRK. "But when we see videos like the band in Ohio, or people in a bar in Thailand skipping and dancing to the song, it is quite tricky to relate to."
He said that the brothers were close to reaching agreement with the unnamed third party who claimed the song infringed copyright, causing it to be pulled off iTunes.
"There is no problem; it's just something that will take time," he said. "There were no agreements, because we were only doing it for fun. You only have to sort out the paperwork when it gets this big."
The Ohio Marching 110 has previously performed versions of other viral hits, such as Gangnam Style by Korea's Psy and Party Rock Anthem by LMFAO.